1. Home
  2. People
  3. Faculty
  4. Eduardo Perozo

Eduardo Perozo

Professor
eperozo@uchicago.edu
Biosciences Graduate Program Association
Publications

  1. State-specific morphological deformations of the lipid bilayer explain mechanosensitive gating of MscS ion channels. Elife. 2023 Jan 30; 12. View in: PubMed

  2. Mechanism of voltage gating in the voltage-sensing phosphatase Ci-VSP. Proc Natl Acad Sci U S A. 2022 11; 119(44):e2206649119. View in: PubMed

  3. Mechanism of voltage sensing in Ca2+- and voltage-activated K+ (BK) channels. Proc Natl Acad Sci U S A. 2022 06 21; 119(25):e2204620119. View in: PubMed

  4. Molecular determinants of inhibition of the human proton channel hHv1 by the designer peptide C6 and a bivalent derivative. Proc Natl Acad Sci U S A. 2022 06 07; 119(23):e2120750119. View in: PubMed

  5. The conformational cycle of prestin underlies outer-hair cell electromotility. Nature. 2021 12; 600(7889):553-558. View in: PubMed

  6. Computational study of non-conductive selectivity filter conformations and C-type inactivation in a voltage-dependent potassium channel. J Gen Physiol. 2021 09 06; 153(9). View in: PubMed

  7. Direct activation of the proton channel by albumin leads to human sperm capacitation and sustained release of inflammatory mediators by neutrophils. Nat Commun. 2021 06 22; 12(1):3855. View in: PubMed

  8. Mechanism of C-type inactivation in the hERG potassium channel. Sci Adv. 2021 01; 7(5). View in: PubMed

  9. Electromechanical coupling in the hyperpolarization-activated K+ channel KAT1. Nature. 2020 07; 583(7814):145-149. View in: PubMed

  10. Molecular basis of force-from-lipids gating in the mechanosensitive channel MscS. Elife. 2019 12 27; 8. View in: PubMed

  11. Real time dynamics of Gating-Related conformational changes in CorA. Elife. 2019 11 27; 8. View in: PubMed

  12. De novo GRIN variants in NMDA receptor M2 channel pore-forming loop are associated with neurological diseases. Hum Mutat. 2019 12; 40(12):2393-2413. View in: PubMed

  13. Role of human Hv1 channels in sperm capacitation and white blood cell respiratory burst established by a designed peptide inhibitor. Proc Natl Acad Sci U S A. 2018 12 11; 115(50):E11847-E11856. View in: PubMed

  14. Rapid constriction of the selectivity filter underlies C-type inactivation in the KcsA potassium channel. J Gen Physiol. 2018 10 01; 150(10):1408-1420. View in: PubMed

  15. Never at rest: insights into the conformational dynamics of ion channels from cryo-electron microscopy. J Physiol. 2018 04 01; 596(7):1107-1119. View in: PubMed

  16. Structural Dynamics of the MscL C-terminal Domain. Sci Rep. 2017 12 08; 7(1):17229. View in: PubMed

  17. The gating cycle of a K+ channel at atomic resolution. Elife. 2017 11 22; 6. View in: PubMed

  18. Chemical substitutions in the selectivity filter of potassium channels do not rule out constricted-like conformations for C-type inactivation. Proc Natl Acad Sci U S A. 2017 10 17; 114(42):11145-11150. View in: PubMed

  19. Towards a Structural View of Drug Binding to hERG K+ Channels. Trends Pharmacol Sci. 2017 10; 38(10):899-907. View in: PubMed

  20. Probing the Effects of Gating on the Ion Occupancy of the K+ Channel Selectivity Filter Using Two-Dimensional Infrared Spectroscopy. J Am Chem Soc. 2017 07 05; 139(26):8837-8845. View in: PubMed

  21. Instantaneous ion configurations in the K+ ion channel selectivity filter revealed by 2D IR spectroscopy. Science. 2016 09 02; 353(6303):1040-1044. View in: PubMed

  22. From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins. Structure. 2016 Oct 04; 24(10):1830-1841. View in: PubMed

  23. Toward a structural blueprint for bilayer-mediated channel mechanosensitivity. Channels (Austin). 2017 03 04; 11(2):91-93. View in: PubMed

  24. The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels. Nat Commun. 2016 06 22; 7:11984. View in: PubMed

  25. Multi-ion free energy landscapes underscore the microscopic mechanism of ion selectivity in the KcsA channel. Biochim Biophys Acta. 2016 Jul; 1858(7 Pt B):1722-32. View in: PubMed

  26. Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating. Cell. 2016 Feb 11; 164(4):747-56. View in: PubMed

  27. Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs. Structure. 2016 Feb 02; 24(2):300-9. View in: PubMed

  28. Resting state of the human proton channel dimer in a lipid bilayer. Proc Natl Acad Sci U S A. 2015 Nov 03; 112(44):E5926-35. View in: PubMed

  29. Biomolecular DNP-Supported NMR Spectroscopy using Site-Directed Spin Labeling. Chemistry. 2015 Sep 07; 21(37):12971-7. View in: PubMed

  30. xMDFF: molecular dynamics flexible fitting of low-resolution X-ray structures. Acta Crystallogr D Biol Crystallogr. 2014 Sep; 70(Pt 9):2344-55. View in: PubMed

  31. Molecular mechanism of Mg2+-dependent gating in CorA. Nat Commun. 2014 Apr 02; 5:3590. View in: PubMed

  32. Conformational dynamics at the inner gate of KcsA during activation. Biochemistry. 2014 Apr 29; 53(16):2557-9. View in: PubMed

  33. A repulsion mechanism explains magnesium permeation and selectivity in CorA. Proc Natl Acad Sci U S A. 2014 Feb 25; 111(8):3002-7. View in: PubMed

  34. Biochemical and structural analysis of the hyperpolarization-activated K(+) channel MVP. Biochemistry. 2014 Mar 18; 53(10):1627-36. View in: PubMed

  35. Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain. Nat Struct Mol Biol. 2014 Mar; 21(3):244-52. View in: PubMed

  36. Dynamics transitions at the outer vestibule of the KcsA potassium channel during gating. Proc Natl Acad Sci U S A. 2014 Feb 04; 111(5):1831-6. View in: PubMed

  37. Structural basis of lipid-driven conformational transitions in the KvAP voltage-sensing domain. Nat Struct Mol Biol. 2014 Feb; 21(2):160-6. View in: PubMed

  38. Recovery from slow inactivation in K+ channels is controlled by water molecules. Nature. 2013 Sep 05; 501(7465):121-4. View in: PubMed

  39. Importance of lipid-pore loop interface for potassium channel structure and function. Proc Natl Acad Sci U S A. 2013 Aug 06; 110(32):13008-13. View in: PubMed

  40. Binding of the CYK-4 subunit of the centralspindlin complex induces a large scale conformational change in the kinesin subunit. J Biol Chem. 2013 Jul 05; 288(27):19785-95. View in: PubMed

  41. An emerging consensus on voltage-dependent gating from computational modeling and molecular dynamics simulations. J Gen Physiol. 2012 Dec; 140(6):587-94. View in: PubMed

  42. Expression, purification, and reconstitution of the voltage-sensing domain from Ci-VSP. Biochemistry. 2012 Oct 16; 51(41):8132-42. View in: PubMed

  43. Symmetry-constrained analysis of pulsed double electron-electron resonance (DEER) spectroscopy reveals the dynamic nature of the KcsA activation gate. J Am Chem Soc. 2012 Oct 03; 134(39):16360-9. View in: PubMed

  44. Mechanism of Cd2+ coordination during slow inactivation in potassium channels. Structure. 2012 Aug 08; 20(8):1332-42. View in: PubMed

  45. Protein conformational dynamics in the mechanism of HIV-1 protease catalysis. Proc Natl Acad Sci U S A. 2011 Dec 27; 108(52):20982-7. View in: PubMed

  46. Thermodynamic coupling between activation and inactivation gating in potassium channels revealed by free energy molecular dynamics simulations. J Gen Physiol. 2011 Dec; 138(6):571-80. View in: PubMed

  47. Molecular coupling in the human ether-a-go-go-related gene-1 (hERG1) K+ channel inactivation pathway. J Biol Chem. 2011 Nov 11; 286(45):39091-9. View in: PubMed

  48. Mechanism of activation gating in the full-length KcsA K+ channel. Proc Natl Acad Sci U S A. 2011 Jul 19; 108(29):11896-9. View in: PubMed

  49. A multipoint hydrogen-bond network underlying KcsA C-type inactivation. Biophys J. 2011 May 18; 100(10):2387-93. View in: PubMed

  50. On the structural basis of modal gating behavior in K(+) channels. Nat Struct Mol Biol. 2011 Jan; 18(1):67-74. View in: PubMed

  51. Up a hydrophobic creek with a short paddle. Cell. 2010 Aug 20; 142(4):515-6. View in: PubMed

  52. Structural dynamics of the magnesium-bound conformation of CorA in a lipid bilayer. Structure. 2010 Jul 14; 18(7):868-78. View in: PubMed

  53. Structural basis for the coupling between activation and inactivation gates in K(+) channels. Nature. 2010 Jul 08; 466(7303):272-5. View in: PubMed

  54. Structural mechanism of C-type inactivation in K(+) channels. Nature. 2010 Jul 08; 466(7303):203-8. View in: PubMed

  55. The activated state of a sodium channel voltage sensor in a membrane environment. Proc Natl Acad Sci U S A. 2010 Mar 23; 107(12):5435-40. View in: PubMed

  56. Design and characterization of a constitutively open KcsA. FEBS Lett. 2010 Mar 19; 584(6):1133-8. View in: PubMed

  57. A molecular mechanism for proton-dependent gating in KcsA. FEBS Lett. 2010 Mar 19; 584(6):1126-32. View in: PubMed

  58. A designer ligand specific for Kv1.3 channels from a scorpion neurotoxin-based library. Proc Natl Acad Sci U S A. 2009 Dec 29; 106(52):22211-6. View in: PubMed

  59. Structural biology: A channel with a twist. Nature. 2009 Sep 03; 461(7260):47-9. View in: PubMed

  60. Crystal structure of full-length KcsA in its closed conformation. Proc Natl Acad Sci U S A. 2009 Apr 21; 106(16):6644-9. View in: PubMed

  61. Dynamics of "flap" structures in three HIV-1 protease/inhibitor complexes probed by total chemical synthesis and pulse-EPR spectroscopy. J Am Chem Soc. 2009 Jan 28; 131(3):884-5. View in: PubMed

  62. Distinct gate conformations of the ABC transporter BtuCD revealed by electron spin resonance spectroscopy and chemical cross-linking. FEBS Lett. 2009 Jan 22; 583(2):266-70. View in: PubMed

  63. A structural mechanism for MscS gating in lipid bilayers. Science. 2008 Aug 29; 321(5893):1210-4. View in: PubMed

  64. Structural refinement of membrane proteins by restrained molecular dynamics and solvent accessibility data. Biophys J. 2008 Dec; 95(11):5349-61. View in: PubMed

  65. Three-dimensional architecture of membrane-embedded MscS in the closed conformation. J Mol Biol. 2008 Apr 18; 378(1):55-70. View in: PubMed

  66. Structural dynamics of an isolated voltage-sensor domain in a lipid bilayer. Structure. 2008 Mar; 16(3):398-409. View in: PubMed

  67. A quantitative description of KcsA gating II: single-channel currents. J Gen Physiol. 2007 Nov; 130(5):479-96. View in: PubMed

  68. A quantitative description of KcsA gating I: macroscopic currents. J Gen Physiol. 2007 Nov; 130(5):465-78. View in: PubMed

  69. Molecular driving forces determining potassium channel slow inactivation. Nat Struct Mol Biol. 2007 Nov; 14(11):1062-9. View in: PubMed

  70. Asymmetry in the structure of the ABC transporter-binding protein complex BtuCD-BtuF. Science. 2007 Sep 07; 317(5843):1387-90. View in: PubMed

  71. An optimized purification and reconstitution method for the MscS channel: strategies for spectroscopical analysis. Biochemistry. 2007 Jun 12; 46(23):6766-73. View in: PubMed

  72. How to gate an ion channel: lessons from MthK. Nat Struct Mol Biol. 2007 Mar; 14(3):180-2. View in: PubMed

  73. Ion conduction through MscS as determined by electrophysiology and simulation. Biophys J. 2007 Feb 01; 92(3):886-902. View in: PubMed

  74. Detection of the opening of the bundle crossing in KcsA with fluorescence lifetime spectroscopy reveals the existence of two gates for ion conduction. J Gen Physiol. 2006 Nov; 128(5):569-81. View in: PubMed

  75. Molecular determinants of gating at the potassium-channel selectivity filter. Nat Struct Mol Biol. 2006 Apr; 13(4):311-8. View in: PubMed

  76. Voltage-dependent gating at the KcsA selectivity filter. Nat Struct Mol Biol. 2006 Apr; 13(4):319-22. View in: PubMed

  77. Gating prokaryotic mechanosensitive channels. Nat Rev Mol Cell Biol. 2006 Feb; 7(2):109-19. View in: PubMed

  78. Explicit treatment of spin labels in modeling of distance constraints from dipolar EPR and DEER. J Am Chem Soc. 2005 Jul 06; 127(26):9334-5. View in: PubMed

  79. Electrostatic interaction of a K+ channel RCK domain with charged membrane surfaces. Biochemistry. 2005 Jan 11; 44(1):62-71. View in: PubMed

  80. Molecular architecture of the KvAP voltage-dependent K+ channel in a lipid bilayer. Science. 2004 Oct 15; 306(5695):491-5. View in: PubMed

  81. Structure and mechanism in prokaryotic mechanosensitive channels. Curr Opin Struct Biol. 2003 Aug; 13(4):432-42. View in: PubMed

  82. The voltage sensor and the gate in ion channels. Adv Protein Chem. 2003; 63:211-41. View in: PubMed

  83. Structural biology. Force and voltage sensors in one structure. Science. 2002 Nov 22; 298(5598):1562-3. View in: PubMed

  84. Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Nature. 2002 Aug 29; 418(6901):942-8. View in: PubMed

  85. Reactions of cysteines substituted in the amphipathic N-terminal tail of a bacterial potassium channel with hydrophilic and hydrophobic maleimides. Proc Natl Acad Sci U S A. 2002 Sep 03; 99(18):11605-10. View in: PubMed

  86. New structural perspectives on K(+) channel gating. Structure. 2002 Aug; 10(8):1027-9. View in: PubMed

  87. Physical principles underlying the transduction of bilayer deformation forces during mechanosensitive channel gating. Nat Struct Biol. 2002 Sep; 9(9):696-703. View in: PubMed

  88. EPR approaches to ion channel structure and function. Novartis Found Symp. 2002; 245:146-58; discussion 158-64, 165-8. View in: PubMed

  89. Calculation of rigid-body conformational changes using restraint-driven Cartesian transformations. Biophys J. 2001 Nov; 81(5):2530-46. View in: PubMed

  90. Structure of the KcsA channel intracellular gate in the open state. Nat Struct Biol. 2001 Oct; 8(10):883-7. View in: PubMed

  91. Site-directed spin-labeling analysis of reconstituted Mscl in the closed state. J Gen Physiol. 2001 Aug; 118(2):193-206. View in: PubMed

  92. Molecular architecture of full-length KcsA: role of cytoplasmic domains in ion permeation and activation gating. J Gen Physiol. 2001 Feb; 117(2):165-80. View in: PubMed

  93. Structure and packing orientation of transmembrane segments in voltage-dependent channels. Lessons from perturbation analysis. J Gen Physiol. 2000 Jan; 115(1):29-32. View in: PubMed

  94. Structural rearrangements underlying K+-channel activation gating. Science. 1999 Jul 02; 285(5424):73-8. View in: PubMed

  95. Shedding light on voltage-dependent gating. J Gen Physiol. 1998 Oct; 112(4):373-6. View in: PubMed

  96. Three-dimensional architecture and gating mechanism of a K+ channel studied by EPR spectroscopy. Nat Struct Biol. 1998 Jun; 5(6):459-69. View in: PubMed

  97. pH-dependent gating in the Streptomyces lividans K+ channel. Biochemistry. 1998 Mar 10; 37(10):3229-36. View in: PubMed

  98. Structural dynamics of the Streptomyces lividans K+ channel (SKC1): secondary structure characterization from FTIR spectroscopy. FEBS Lett. 1998 Feb 20; 423(2):205-12. View in: PubMed

  99. Structural dynamics of the Streptomyces lividans K+ channel (SKC1): oligomeric stoichiometry and stability. Biochemistry. 1997 Aug 19; 36(33):10343-52. View in: PubMed

  100. Voltage activation of reconstituted sodium channels: use of bacteriorhodopsin as a light-driven current source. Biochemistry. 1993 Oct 05; 32(39):10471-8. View in: PubMed

  101. Gating currents from a nonconducting mutant reveal open-closed conformations in Shaker K+ channels. Neuron. 1993 Aug; 11(2):353-8. View in: PubMed

  102. Purification and reconstitution of functional Shaker K+ channels assayed with a light-driven voltage-control system. Biochemistry. 1994 Feb 15; 33(6):1295-9. View in: PubMed

  103. S4 mutations alter gating currents of Shaker K channels. Biophys J. 1994 Feb; 66(2 Pt 1):345-54. View in: PubMed

  104. Gating of Shaker K+ channels: I. Ionic and gating currents. Biophys J. 1994 Apr; 66(4):996-1010. View in: PubMed

  105. Gating of Shaker K+ channels: II. The components of gating currents and a model of channel activation. Biophys J. 1994 Apr; 66(4):1011-21. View in: PubMed

  106. Removal of transducer HtrI allows electrogenic proton translocation by sensory rhodopsin I. Proc Natl Acad Sci U S A. 1994 Oct 11; 91(21):10188-92. View in: PubMed

  107. Chemical modification of squid axon K+ channel -SH groups with the organic mercurial compound p-hydroxymercuriphenylsulfonic acid (PHMPS). Pflugers Arch. 1994 Oct; 428(3-4):315-22. View in: PubMed

  108. Spin-labeled amphotericin B: synthesis, characterization, biological and spectroscopic properties. Biochim Biophys Acta. 1987 Mar 12; 897(3):467-73. View in: PubMed

  109. Modulation of K channels in dialyzed squid axons. ATP-mediated phosphorylation. J Gen Physiol. 1989 Jun; 93(6):1195-218. View in: PubMed

  110. Phosphorylation affects voltage gating of the delayed rectifier K+ channel by electrostatic interactions. Neuron. 1990 Nov; 5(5):685-90. View in: PubMed

  111. Molecular basis of gating charge immobilization in Shaker potassium channels. Science. 1991 Nov 01; 254(5032):679-83. View in: PubMed

  112. Single channel studies of the phosphorylation of K+ channels in the squid giant axon. II. Nonstationary conditions. J Gen Physiol. 1991 Jul; 98(1):19-34. View in: PubMed

  113. Single channel studies of the phosphorylation of K+ channels in the squid giant axon. I. Steady-state conditions. J Gen Physiol. 1991 Jul; 98(1):1-17. View in: PubMed

  114. Phosphorylation of K+ channels in the squid giant axon. A mechanistic analysis. J Bioenerg Biomembr. 1991 Aug; 23(4):599-613. View in: PubMed

  115. Gating currents in Shaker K+ channels. Implications for activation and inactivation models. Biophys J. 1992 Apr; 62(1):160-8; discussion 169-71. View in: PubMed
© 2023 The University of Chicago | All Rights Reserved.
Instagram Twitter