D. Allan Drummond

Associate Professor
Websites
Research Summary
Dr. Drummond is an Associate Professor of Biochemistry & Molecular Biology and Human Genetics. His group studies how cells respond to stress at the molecular level, focusing on formation and dissolution of large assemblies of proteins and RNA during and after stresses such as heat shock. Drummond's lab uses a wide range of techniques, including in vivo imaging, in vitro reconstitution and mechanistic biochemistry, quantitative proteomics, and molecular evolutionary analyses. Because many features of stress-triggered assembly processes are shared across eukaryotes, including humans, the group employs budding yeast, Saccharomyces cerevisiae, as a model organism.
Keywords
Cell Biology, Stress Response, Physiological, Heat Shock Response, Phase Separation, Molecular Evolution
Education
  • Princeton University, Princeton, NJ, B.S.E. Mechanical and aerospace engineering 06/1995
  • California Institute of Technology, Pasadena, CA, Ph.D. Computation and neural systems 06/2006
  • Harvard University, Cambridge, MA, Systems biology 10/2011
Awards & Honors
  • 2006 - 2011 Bauer Fellow Harvard University
  • 2012 - 2014 Sloan Research Fellow Alfred P. Sloan Foundation
  • 2012 - 2016 Pew Scholar in the Biomedical Sciences Pew Charitable Trusts
Publications
  1. Triandafillou CG, Katanski CD, Dinner AR, Drummond DA. Transient intracellular acidification regulates the core transcriptional heat shock response. Elife. 2020 Aug 07; 9. View in: PubMed

  2. Yoo H, Triandafillou C, Drummond DA. Cellular sensing by phase separation: Using the process, not just the products. J Biol Chem. 2019 05 03; 294(18):7151-7159. View in: PubMed

  3. McCully AL, Behringer MG, Gliessman JR, Pilipenko EV, Mazny JL, Lynch M, Drummond DA, McKinlay JB. An Escherichia coli Nitrogen Starvation Response Is Important for Mutualistic Coexistence with Rhodopseudomonas palustris. Appl Environ Microbiol. 2018 07 15; 84(14). View in: PubMed

  4. Riback JA, Katanski CD, Kear-Scott JL, Pilipenko EV, Rojek AE, Sosnick TR, Drummond DA. Stress-Triggered Phase Separation Is an Adaptive, Evolutionarily Tuned Response. Cell. 2017 03 09; 168(6):1028-1040.e19. View in: PubMed

  5. Triandafillou CG, Drummond DA. Heat Shock Factor 1: From Fire Chief to Crowd-Control Specialist. Mol Cell. 2016 07 07; 63(1):1-2. View in: PubMed

  6. Wallace EW, Kear-Scott JL, Pilipenko EV, Schwartz MH, Laskowski PR, Rojek AE, Katanski CD, Riback JA, Dion MF, Franks AM, Airoldi EM, Pan T, Budnik BA, Drummond DA. Reversible, Specific, Active Aggregates of Endogenous Proteins Assemble upon Heat Stress. Cell. 2015 Sep 10; 162(6):1286-98. View in: PubMed

  7. Wallace EW, Drummond DA. Dying mRNA Tells a Story of Its Life. Cell. 2015 Jun 04; 161(6):1246-8. View in: PubMed

  8. Bhojani N, Paonessa JE, Hameed TA, Worcester EM, Evan AP, Coe FL, Borofsky MS, Lingeman JE. Nephrocalcinosis in Calcium Stone Formers Who Do Not have Systemic Disease. J Urol. 2015 Nov; 194(5):1308-12. View in: PubMed

  9. Franks AM, Csárdi G, Drummond DA, Airoldi EM. Estimating a structured covariance matrix from multi-lab measurements in high-throughput biology. J Am Stat Assoc. 2015 Mar 01; 110(509):27-44. View in: PubMed

  10. Csárdi G, Franks A, Choi DS, Airoldi EM, Drummond DA. Accounting for experimental noise reveals that mRNA levels, amplified by post-transcriptional processes, largely determine steady-state protein levels in yeast. PLoS Genet. 2015 May; 11(5):e1005206. View in: PubMed

  11. Ko B, Bergsland K, Gillen DL, Evan AP, Clark DL, Baylock J, Coe FL, Worcester EM. Sex differences in proximal and distal nephron function contribute to the mechanism of idiopathic hypercalcuria in calcium stone formers. Am J Physiol Regul Integr Comp Physiol. 2015 Jul 01; 309(1):R85-92. View in: PubMed

  12. Zaborske JM, Bauer DuMont VL, Wallace EW, Pan T, Aquadro CF, Drummond DA. Correction: A nutrient-driven tRNA modification alters translational fidelity and genome-wide protein coding across an animal genus. PLoS Biol. 2015 Apr; 13(4):e1002150. View in: PubMed

  13. Ennis JL, Worcester EM, Coe FL, Sprague SM. Current recommended 25-hydroxyvitamin D targets for chronic kidney disease management may be too low. J Nephrol. 2016 Feb; 29(1):63-70. View in: PubMed

  14. Kang MH, Wang J, Makena MR, Lee JS, Paz N, Hall CP, Song MM, Calderon RI, Cruz RE, Hindle A, Ko W, Fitzgerald JB, Drummond DC, Triche TJ, Reynolds CP. Activity of MM-398, nanoliposomal irinotecan (nal-IRI), in Ewing's family tumor xenografts is associated with high exposure of tumor to drug and high SLFN11 expression. Clin Cancer Res. 2015 Mar 01; 21(5):1139-50. View in: PubMed

  15. Evan AP, Coe FL, Connors BA, Handa RK, Lingeman JE, Worcester EM. Mechanism by which shock wave lithotripsy can promote formation of human calcium phosphate stones. Am J Physiol Renal Physiol. 2015 Apr 15; 308(8):F938-49. View in: PubMed

  16. Evan AP, Worcester EM, Williams JC, Sommer AJ, Lingeman JE, Phillips CL, Coe FL. Biopsy proven medullary sponge kidney: clinical findings, histopathology, and role of osteogenesis in stone and plaque formation. Anat Rec (Hoboken). 2015 May; 298(5):865-77. View in: PubMed

  17. Zaborske JM, DuMont VL, Wallace EW, Pan T, Aquadro CF, Drummond DA. A nutrient-driven tRNA modification alters translational fidelity and genome-wide protein coding across an animal genus. PLoS Biol. 2014 Dec; 12(12):e1002015. View in: PubMed

  18. Evan AP, Worcester EM, Coe FL, Williams J, Lingeman JE. Mechanisms of human kidney stone formation. Urolithiasis. 2015 Jan; 43 Suppl 1:19-32. View in: PubMed

  19. Williams JC, Lingeman JE, Coe FL, Worcester EM, Evan AP. Micro-CT imaging of Randall's plaques. Urolithiasis. 2015 Jan; 43 Suppl 1:13-7. View in: PubMed

  20. Evan AP, Lingeman JE, Worcester EM, Sommer AJ, Phillips CL, Williams JC, Coe FL. Contrasting histopathology and crystal deposits in kidneys of idiopathic stone formers who produce hydroxy apatite, brushite, or calcium oxalate stones. Anat Rec (Hoboken). 2014 Apr; 297(4):731-48. View in: PubMed

  21. Worcester EM, Evan AP, Coe FL, Lingeman JE, Krambeck A, Sommers A, Phillips CL, Milliner D. A test of the hypothesis that oxalate secretion produces proximal tubule crystallization in primary hyperoxaluria type I. Am J Physiol Renal Physiol. 2013 Dec 01; 305(11):F1574-84. View in: PubMed

  22. Geiler-Samerotte KA, Hashimoto T, Dion MF, Budnik BA, Airoldi EM, Drummond DA. Quantifying condition-dependent intracellular protein levels enables high-precision fitness estimates. PLoS One. 2013; 8(9):e75320. View in: PubMed

  23. Worcester EM, Bergsland KJ, Gillen DL, Coe FL. Evidence for increased renal tubule and parathyroid gland sensitivity to serum calcium in human idiopathic hypercalciuria. Am J Physiol Renal Physiol. 2013 Sep 15; 305(6):F853-60. View in: PubMed

  24. Bergsland KJ, Worcester EM, Coe FL. Role of proximal tubule in the hypocalciuric response to thiazide of patients with idiopathic hypercalciuria. Am J Physiol Renal Physiol. 2013 Aug 15; 305(4):F592-9. View in: PubMed

  25. Wallace EW, Airoldi EM, Drummond DA. Estimating selection on synonymous codon usage from noisy experimental data. Mol Biol Evol. 2013 Jun; 30(6):1438-53. View in: PubMed

  26. Drummond DA. How infidelity creates a sticky situation. Mol Cell. 2012 Dec 14; 48(5):663-4. View in: PubMed

  27. Agashe D, Martinez-Gomez NC, Drummond DA, Marx CJ. Good codons, bad transcript: large reductions in gene expression and fitness arising from synonymous mutations in a key enzyme. Mol Biol Evol. 2013 Mar; 30(3):549-60. View in: PubMed

  28. Geiler-Samerotte KA, Dion MF, Budnik BA, Wang SM, Hartl DL, Drummond DA. Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast. Proc Natl Acad Sci U S A. 2011 Jan 11; 108(2):680-5. View in: PubMed

  29. Wilke CO, Drummond DA. Signatures of protein biophysics in coding sequence evolution. Curr Opin Struct Biol. 2010 Jun; 20(3):385-9. View in: PubMed

  30. Drummond DA. Protein evolution: innovative chaps. Curr Biol. 2009 Sep 15; 19(17):R740-2. View in: PubMed

  31. Drummond DA, Wilke CO. The evolutionary consequences of erroneous protein synthesis. Nat Rev Genet. 2009 Oct; 10(10):715-24. View in: PubMed

  32. Drummond DA, Wilke CO. Mistranslation-induced protein misfolding as a dominant constraint on coding-sequence evolution. Cell. 2008 Jul 25; 134(2):341-52. View in: PubMed

  33. Zhou T, Drummond DA, Wilke CO. Contact density affects protein evolutionary rate from bacteria to animals. J Mol Evol. 2008 Apr; 66(4):395-404. View in: PubMed

  34. Li Y, Drummond DA, Sawayama AM, Snow CD, Bloom JD, Arnold FH. A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments. Nat Biotechnol. 2007 Sep; 25(9):1051-6. View in: PubMed

  35. Zhou Y, Drummond DC, Zou H, Hayes ME, Adams GP, Kirpotin DB, Marks JD. Impact of single-chain Fv antibody fragment affinity on nanoparticle targeting of epidermal growth factor receptor-expressing tumor cells. J Mol Biol. 2007 Aug 24; 371(4):934-47. View in: PubMed

  36. Bloom JD, Drummond DA, Arnold FH, Wilke CO. Structural determinants of the rate of protein evolution in yeast. Mol Biol Evol. 2006 Sep; 23(9):1751-61. View in: PubMed

  37. Wilke CO, Drummond DA. Population genetics of translational robustness. Genetics. 2006 May; 173(1):473-81. View in: PubMed

  38. Drummond DA, Raval A, Wilke CO. A single determinant dominates the rate of yeast protein evolution. Mol Biol Evol. 2006 Feb; 23(2):327-37. View in: PubMed

  39. Drummond DA, Bloom JD, Adami C, Wilke CO, Arnold FH. Why highly expressed proteins evolve slowly. Proc Natl Acad Sci U S A. 2005 Oct 04; 102(40):14338-43. View in: PubMed

  40. Wilke CO, Bloom JD, Drummond DA, Raval A. Predicting the tolerance of proteins to random amino acid substitution. Biophys J. 2005 Dec; 89(6):3714-20. View in: PubMed

  41. Drummond DA, Iverson BL, Georgiou G, Arnold FH. Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins. J Mol Biol. 2005 Jul 22; 350(4):806-16. View in: PubMed

  42. Drummond DA, Silberg JJ, Meyer MM, Wilke CO, Arnold FH. On the conservative nature of intragenic recombination. Proc Natl Acad Sci U S A. 2005 Apr 12; 102(15):5380-5. View in: PubMed

  43. Bloom JD, Silberg JJ, Wilke CO, Drummond DA, Adami C, Arnold FH. Thermodynamic prediction of protein neutrality. Proc Natl Acad Sci U S A. 2005 Jan 18; 102(3):606-11. View in: PubMed