Anthony Kossiakoff, PhD

The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.

California Institute of Technology
Postdoc - Structural Biology
1975

University of Delaware
Ph.D - Physical Chemistry
1972

Davis and Elkins College
BS - Chemistry/Math
1968

Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme binding.
Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme binding. Structure. 2024 Jan 30.
PMID: 38325368

The Molecular Basis of Human ALKBH3 Mediated RNA N1 -methyladenosine (m1 A) Demethylation.
The Molecular Basis of Human ALKBH3 Mediated RNA N1 -methyladenosine (m1 A) Demethylation. Angew Chem Int Ed Engl. 2024 Feb 12; 63(7):e202313900.
PMID: 38158383

Engineered antigen-binding fragments for enhanced crystallization of antibody:antigen complexes.
Engineered antigen-binding fragments for enhanced crystallization of antibody:antigen complexes. Protein Sci. 2024 Jan; 33(1):e4824.
PMID: 37945533

The Molecular Basis of Human ALKBH3 Mediated RNA N1 -methyladenosine (m1 A) Demethylation.
The Molecular Basis of Human ALKBH3 Mediated RNA N1 -methyladenosine (m1 A) Demethylation. Angew Chem Int Ed Engl. 2023 Dec 29; e202313900.
PMID: 38158383

Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes.
Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes. Protein Sci. 2023 Nov 09; e4824.
PMID: 37945533

Structural and molecular basis of choline uptake into the brain by FLVCR2.
Structural and molecular basis of choline uptake into the brain by FLVCR2. bioRxiv. 2023 Oct 05.
PMID: 37873173

Structure of human drug transporters OATP1B1 and OATP1B3.
Structure of human drug transporters OATP1B1 and OATP1B3. Nat Commun. 2023 09 18; 14(1):5774.
PMID: 37723174

Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake.
Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake. bioRxiv. 2023 Aug 01.
PMID: 37577460

Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry.
Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry. Nat Commun. 2023 07 20; 14(1):4368.
PMID: 37474505

ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding.
ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding. bioRxiv. 2023 Jul 19.
PMID: 37502840

View All Publications

Christian Anfinsen Award
Protein Society
2019

Fellow of American Association for Advancement of Science
AAAS
2012

Honorary Degree Doctor of Science
Davis and Elkins College
2011

Alumni Hall of Fame
University of Delaware
2003

Chair Department of Biochemistry and Molecular Biology
University of Chicago
1998 - 2011

Director Institute for Biophysical Dynamics
University of Chicago
1998 - 2003

Director Department of Protein Engineering
Genentech, Inc.
1983 - 1998