The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.
California Institute of Technology
Postdoc - Structural Biology
1975
University of Delaware
Ph.D - Physical Chemistry
1972
Davis and Elkins College
BS - Chemistry/Math
1968
Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to its Human Receptor, Claudin-4.
Cryo-EM Structures of Clostridium perfringens Enterotoxin Bound to its Human Receptor, Claudin-4. bioRxiv. 2024 Jul 12.
PMID: 39026804
Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage.
Structural Insights into the Roles of PARP4 and NAD + in the Human Vault Cage. bioRxiv. 2024 Jun 27.
PMID: 38979142
Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment.
Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment. Commun Biol. 2024 Jun 17; 7(1):733.
PMID: 38886509
Development of Luminescent Biosensors for Calprotectin.
Development of Luminescent Biosensors for Calprotectin. ACS Chem Biol. 2024 Jun 21; 19(6):1250-1259.
PMID: 38843544
Structural and molecular basis of choline uptake into the brain by FLVCR2.
Structural and molecular basis of choline uptake into the brain by FLVCR2. Nature. 2024 May; 629(8012):704-709.
PMID: 38693257
Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP.
Structure of antiviral drug bulevirtide bound to hepatitis B and D virus receptor protein NTCP. Nat Commun. 2024 Mar 20; 15(1):2476.
PMID: 38509088
Structural analysis and conformational dynamics of a holo-adhesion GPCR reveal interplay between extracellular and transmembrane domains.
Structural analysis and conformational dynamics of a holo-adhesion GPCR reveal interplay between extracellular and transmembrane domains. bioRxiv. 2024 Feb 26.
PMID: 38464178
Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme binding.
Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme binding. Structure. 2024 Apr 04; 32(4):411-423.e6.
PMID: 38325368
The Molecular Basis of Human ALKBH3 Mediated RNA N1 -methyladenosine (m1 A) Demethylation.
The Molecular Basis of Human ALKBH3 Mediated RNA N1 -methyladenosine (m1 A) Demethylation. Angew Chem Int Ed Engl. 2024 Feb 12; 63(7):e202313900.
PMID: 38158383
Engineered antigen-binding fragments for enhanced crystallization of antibody:antigen complexes.
Engineered antigen-binding fragments for enhanced crystallization of antibody:antigen complexes. Protein Sci. 2024 Jan; 33(1):e4824.
PMID: 37945533
Christian Anfinsen Award
Protein Society
2019
Fellow of American Association for Advancement of Science
AAAS
2012
Honorary Degree Doctor of Science
Davis and Elkins College
2011
Alumni Hall of Fame
University of Delaware
2003
Chair Department of Biochemistry and Molecular Biology
University of Chicago
1998 - 2011
Director Institute for Biophysical Dynamics
University of Chicago
1998 - 2003
Director Department of Protein Engineering
Genentech, Inc.
1983 - 1998